Effects of parathyroid hormone-related protein on osteogenic and adipogenic differentiation of human mesenchymal stem cells
K. Zhang, F.-j. Zhang, W.-j. Zhao, G.-s. Xing, X. Bai, Y. Wang Department of Laboratory, Tianjin Hospital, Tianjin, China. guoshengxingcn@126.com
OBJECTIVE: This work aims to investigate the effects of parathyroid hormone-related peptide (PTHrP) (1-86) on osteogenic and adipogenic differentiation of human mesenchymal stem cells (hMSCs) and the related mechanisms.
MATERIALS AND METHODS: hMSCs were isolated and cultured in vitro. They were divided into control group, osteogenesis group, adipogenesis group, osteogenesis+PTHrP group and adipogenesis+PTHrP group. The cell proliferation and differentiation, and expression levels of osteopontin (OPN) and lipoprotein lipase (LPL) mRNA were observed.
RESULTS: The proliferation rates of hMSCs in osteogenesis+PTHrP and adipogenesis+PTHrP group were significantly higher than that in control group, respectively (p < 0.01). The alkaline phosphatase (ALP)-positive osteoblasts firstly appeared in osteogenesis+PTHrP group, and Sudan IV-positive adipocytes firstly appeared in adipogenesis group. The expression level of OPN mRNA in osteogenesis+PTHrP group was significantly higher than that in osteogenesis group (p < 0.05), and that in adipogenesis+PTHrP group was also higher than adipogenesis group (p < 0.05). The expression level of LPL mRNA in osteogenesis+PTHrP group was significantly lower than that in osteogenesis group, and that in adipogenesis+PTHrP group was also lower than adipogenesis group (p < 0.05).
CONCLUSIONS: The osteogenesis and adipogenesis are related to each other during the induced differentiation of hMSCs. PTHrP (1-86) can promote the osteogenic differentiation and inhibits the adipogenic differentiation for hMSCs.
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To cite this article
K. Zhang, F.-j. Zhang, W.-j. Zhao, G.-s. Xing, X. Bai, Y. Wang
Effects of parathyroid hormone-related protein on osteogenic and adipogenic differentiation of human mesenchymal stem cells
Eur Rev Med Pharmacol Sci
Year: 2014
Vol. 18 - N. 11
Pages: 1610-1617