Protective effect of minocycline on dexamethasone induced testicular germ cell apoptosis in mice
M. Orazizadeh, M. Hashemitabar, L. Khorsandi Department of Anatomical Sciences, Faculty of Medicine, Ahwaz Jundi-Shapour University of Medical Sciences, Ahwaz (Iran)
Background: Apoptosis involves in testicular germ cell loss in animals and humans, and plays an important role in male fertility. Previous studies have reported neuroprotective and antiapoptotic effects of minocycline.
Aim: In this study the protective effect of Minocycline on testicular germ cell apoptosis arising from dexamethasone (Dex) has been evaluated. Dex is a widely used as a glucocorticoid (GC) agent that its apoptotic effect has been shown.
Materials and Methods: Experimental groups of 8 male mice received one of the following treatments daily for 7 days: 100 mg/kg Minocycline, 7 mg/kg Dex and 7 mg/kg Dex +100 mg/kg Minocycline. Control group was treated with 0.5 ml saline given orally for a week. Then the mice were sacrificed, and their testes processed for assessment of germ cell apoptosis (TUNEL method), the quality of spermatogenesis (Johnsen score system) and testicular sperm counts.
Results: Germ cell apoptosis were significantly increased in Dex treated mice compared with control (P Conclusion: The application of Minocycline may serve as a beneficial medication to protect germ cells against apoptosis.
Corresponding Author: Khorsandi Layasadat, MD; e-mail: layasadat@yahoo.com
![Creative Commons License](https://i.creativecommons.org/l/by-nc-nd/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
To cite this article
M. Orazizadeh, M. Hashemitabar, L. Khorsandi
Protective effect of minocycline on dexamethasone induced testicular germ cell apoptosis in mice
Eur Rev Med Pharmacol Sci
Year: 2009
Vol. 13 - N. 1
Pages: 1-5