LINC00887 regulates the proliferation of nasopharyngeal carcinoma via targeting miRNA-203b-3p to upregulate NUP205

W.-J. Yue, Y. Wang, W.-Y. Li, Z.-D. Wang

Department of Otorhinolaryngology, The Second Affiliated Hospital, Mudanjiang College of Medicine, Mudanjiang, China. m13694537343@163.com

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• Oncology

OBJECTIVE: The purpose of this study was to uncover the regulatory effect of LINC00887 on the progression of nasopharyngeal carcinoma (NPC) and the underlying mechanism.

PATIENTS AND METHODS: Relative level of LINC00887 in NPC tissues and cells was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Thereafter, the regulatory effect of LINC00887 on proliferative ability in SUNE-1 and HK-1 cells was examined by cell counting kit-8 (CCK-8) and 5-Ethynyl-2’-deoxyuridine (EdU) assay. Through Dual-Luciferase reporter gene assay and RNA-Binding Protein Immunoprecipitation (RIP) assay, the interaction in the regulatory loop LINC00887/miRNA-203b-3p/NUP205 was ascertained. At last, rescue experiments were conducted to clarify the involvement of the regulatory loop LINC00887/miRNA-203b-3p/NUP205 in the progression of NPC.

RESULTS: Results showed that LINC00887 was upregulated in NPC tissues and cells, and its overexpression markedly stimulated the proliferative ability in NPC cells. In addition, a potential interaction in the regulatory loop LINC00887/miRNA-203b-3p/NUP205 was discovered, which was responsible for promoting the proliferative ability in NPC.

CONCLUSIONS: LINC00887 promotes the proliferative ability in NPC via absorbing miRNA-203b-3p to upregulate NUP205.

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