Research on the relativity between gene polymorphism and children cardiac insufficiency

X.-H. He, C.-L. Li, N. Ling, Q.-W. Wang, Z.-Z. Wang, X.-J. An

Department of Cardiology, Xuzhou Children’s Hospital, Xuzhou, Jiangsu Province, China. anxinjian001@163.com

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• Cardiology

OBJECTIVE: We analyzed the relationship between Mink-S27 gene polymorphism and children with cardiac insufficiency.

PATIENTS AND METHODS: From April 2013 to April 2015, we enrolled 73 cases of children with cardiac insufficiency for this study, and all 73 were placed in the observation group. 76 normal cases were selected for the control group. Restriction fragment length polymorphism (RFLP) was used to make polymorphism analysis of the Mink-S27.

RESULTS: Our results showed no significant differences in Mink-S27 genotype and allele distribution in both observation and control groups (p>0.05). In lesion samples collected from children with cardiac insufficiency, we detected significant difference in AA, CC genotype frequency and allele frequency between the observation group and the control group (p< 0.05) (X2 = 15.43, p<0.05; X2  = 16.27, p<0.05). Further studies on samples obtained from both groups revealed certain differences of AA, CC, AC genotype frequency and allele frequency in the observation group. The proportion of homozygote (AA, CC) in children with severe cardiac insufficiency was relatively high.

CONCLUSIONS: GNAS2 gene polymorphism was associated with the prevalence of cardiac insufficiency in children. And also the patients’ condition was correlated to the frequency of different genotypes and alleles.

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